Development of Smart Potentiometric Sensors Covered with Non-Biofouling Layer for Detection of Early Stages of Inflammatory Processes Around Joint Replacements

One of the problems occurring after joint replacement is connected with post-surgery infections which are caused by bacteria that form biofilms on surfaces and referred to as biofilm-related (BRI). It also worth noting due bioresponse, strong pathophysiological changes in microclimate an affected surface occur (decrease pH, formation various reactive oxygen species (ROS), depletion Fe ions, increase concentration Ca ions). In this work we have prepared a robust selective potentiometric sensor ROS pH for detection sterile inflammation bacterial fungal infections. The consists conductive polymer layer based polythiophene incorporated porphyrin-metal complex potentiometrically detects presence (H2O2 ClO– This covalent bonds non-biofouling poly(2-methyl-2-oxazoline), works bio-compa­tibilizer. was shown shows rapid response hydrogen peroxide, does not record any interference bovine serum albumin model protein, able fully reversibly detect linear within wide range biological relevant concentrations (from 0.05 μM 10 μM). sensing electrode polyaniline poly(2-methyl-2-oxazoline) titanium alloy support developed peri-implant enable early aforementioned pathologies. electrodes show changing between 5 8 individual pathogenic or yeast, Nernstian slope −59.6/pH.